Determination of dietary fiber content by high-throughput tissue grinder
Dietary fiber is an indispensable “seventh nutrient†for our health. It is more comprehensive in its understanding of physiological functions, but it is relatively unpopular in the detection of content. At present, most of the tests for dietary fiber use enzyme weight method, which has complicated pretreatment, complicated procedures, poor reproducibility, and long detection period. Therefore, it is imperative to establish a fast, simple and accurate method for the detection of dietary fiber. The role of high-throughput tissue grinders in the preparation of samples during the research process cannot be ignored.
As an important part of food nutrition label, dietary fiber has become an important technical indicator for food quality inspection. Many methods have been established for the detection of dietary fiber in foods at home and abroad, such as detergents, enzyme-chemicals, and enzyme-gravimetry. The enzyme-gravimetric method is currently recognized as a method for determining the content of various dietary fibers. Using this method to detect dietary fiber values ​​in foods with accuracy, precision, and linearity, the results are within the allowable range of variability, indicating that the enzyme-gravimetric method has good reproducibility and is a good and reliable method of measurement.
At present, the testing status of the major testing organizations in the country is still at the stage of exploration. Because the detection process mainly relies on manual operation, there are many inspection operation steps, and the repeatability is poor, so accurate quantification cannot be performed. In addition, there are doubts about the regulations of germanium in national standards: the particle size of domestic G2 germanium is 30 μm to 50 μm, which conflicts with the specified 40 μm to 60 μm. At present, most inspection agencies use G2, but some have adopted a particle size of 30 μm to 70 μm. FOSS dietary fiber analyzers have been purchased by a few inspection agencies. They are randomly selected from P280 μm to 120 μm. The borehole aperture problem may directly lead to errors in the experimental results.
Using high-throughput tissue grinder sampling, the dietary fiber analyzer used in the three-dimensional filter bag method adopts high-tech three-dimensional filter bag technology, and for the first time, automated process control such as on-line enzymatic hydrolysis, pH control, temperature monitoring, and high-speed filtration are realized. The manual operation steps are greatly reduced, reagent pollution and manpower are reduced, inspection efficiency is improved, repeatability and stability are increased, and inspection data are more accurate and scientific.
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